Conophylline inhibits high fat diet-induced non-alcoholic fatty liver disease in mice
Fig 3
Representative images of the liver stained with Oil Red O and hepatic TG contents.
Mice were fed with a control diet (Control, n = 5) (A), high-fat diet (HFD, n = 7) (B), HFD with conophylline [HFD + CnP (0.5 μg/g), n = 4] (C), or HFD with conophylline [HFD + CnP (1 μg/g), n = 4] (D). Original magnification, 200X. (E) Oil red O positive area was quantified in 5 randomly selected fields per section. The percentage of Oil Red O-positive area was measured using a computerized image analysis system with Image-Pro Plus version 4.5 (Media Cybernetics, Silver Spring, MD, USA).(F) Hepatic TG contents (mg/dL) in the respective groups. Statistical analysis was performed using ANOVA, and the data are expressed as means ± SE.