Deletion of genes involved in the ketogluconate metabolism, Entner-Doudoroff pathway, and glucose dehydrogenase increase local and invasive virulence phenotypes in Streptococcus pneumoniae
Fig 7
FISH of chinchilla lung and brain necropsy specimens following transbullar infection with the SP 0320 mutant strain.
16S rRNA gene specific probe labeled with pneumococcal specific Cy3 and FAM was hybridized in the presence of formamide to the tissue samples. Green = pneumococcal specific; Red = eubacterial generic; yellow = overlay of the two dyes. The animal died three days post infection.