Involvement of C-terminal truncation mutation of kinesin-5 in resistance to kinesin-5 inhibitor

doi:10.1371/journal.pone.0209296

Involvement of C-terminal truncation mutation of kinesin-5 in resistance to kinesin-5 inhibitor

Fig 8

Localization of HA-tagged and bulk KIF11 on mitotic spindle.

KIF11^WT-HA or KIF11^S1017fs-HA transfected HeLa cells were treated with 4 μM STLC and 10 μM MG132 for 5 h, and the HA-tagged and the bulk KIF11 localization was examined using immunofluorescence microscopy. Mitotic spindle assembly of the cells expressing KIF11^S1017fs-HA showed a moderate STLC resistance, and KIF11^S1017fs-HA showed a higher accumulation on bipolar spindle than bulk KIF11 in the presence of STLC. Scale bar is 10 μm.

doi: https://doi.org/10.1371/journal.pone.0209296.g008