ERK-mediated TIMELESS expression suppresses G2/M arrest in colon cancer cells
Fig 6
TIMELESS depletion sensitizes colon cancer cells to Wee1 and CHK1 inhibition.
Cell metabolic capacity was measured in a panel of colon cancer cells following RNAi-mediated depletion of TIMELESS with Wee1 inhibition or CHK1 inhibition using alamarBlue assays 96 hours after transfection. 300 nM of Wee1 (MK-1775) or CHK1 (AZD7762) inhibitor was added 48 hours after transfection. Data are normalized to the DMSO treated control transfection (far left bar). Data are shown as mean ± SD and are representative of four or five independent experiments for each condition and cell line. The lower case letters denote a statistical significance (one-way ANOVA with Bonferroni’s Multiple Comparison test for the specified comparisons) with a p value less than 0.001 for the following comparisons: a—DMSO-treated siCont vs DMSO-treated siTIM; b—MK-1775-treated siCont vs MK-1775-treated siTIM; c—AZD7762-treated siCont vs AZD7762-treated siTIM; d—DMSO-treated siTIM vs MK-1775-treated siTIM; e—DMSO-treated siTIM vs AZD7762-treated siTIM.