Trynity controls epidermal barrier function and respiratory tube maturation in Drosophila by modulating apical extracellular matrix nano-patterning
Fig 1
tyn mutants and their gross phenotypes.
(A) Tyn protein structure and tyn DNA sequence of the region including the CRISPR/Cas9 target site. Tyn has 3 PAN/Apple domains, a ZP domain, and a transmembrane domain. The target site is indicated by an arrowhead in the diagram, and the sequence is underlined. tyn1 and tyn2 contained 23- and 4-base deletions respectively, which resulted in early stop codons (red letters). (B) Larval body size for each genotype. Day 1 means ~24 hours after hatching. On days 2 and 3, the tyn mutants remained small, while control larvae (Oregon R and y2, cho2, v1) grew larger. Scale bar: 500 μm. (C) On day 3, the tyn1 mutant larvae lacked anterior spiracles, while those of Oregon R were obvious (arrowheads). Scale bar: 50 μm. (D) Larval viability of each genotype. n = 100 for each. (E) Photographs of larvae with yeast paste. Arrowheads indicate larvae that stayed at the periphery of the yeast paste without entering it. In the left panel, there were 5 larvae within the food. The quantified data are shown in (F). n = 15–20 for each. **P<0.01, *P<0.05 by Fisher’s exact test with Benjamini & Hochberg correction.