Sphingosine 1-phosphate (S1P) reduces hepatocyte growth factor-induced migration of hepatocellular carcinoma cells via S1P receptor 2
Fig 6
Effect of JTE013 on the suppression by S1P of the HGF-induced migration of HuH7 cells.
The cells were pretreated with 1 μM of JTE013 for 10 min prior to 10 μM of S1P treatment for 60 min, and then stimulated by 30 ng/ml of HGF or vehicle for 23 h. The migrated cells were fixed with paraformaldehyde, and stained with DAPI for nucleus (blue signal). The cells were photographed by fluorescent microscopy at a magnification of 20× (upper panel) and counted (bar panel). Each value represents the mean ± SD of triplicate determinations from three independent cell preparations. *p<0.05 compared to the value of the control cells without HGF. **p<0.05 compared to the value of the cells with HGF alone. ***p<0.05 compared to the value of the cells with HGF and S1P pretreatment. N.S. designates no significant difference between the indicated pairs. Scale bar: 100 μm.