Assessing the role of the T-box transcription factor Eomes in B cell differentiation during either Th1 or Th2 cell-biased responses
Fig 5
Eomes is not detected in germinal center B cells during either Th1 or Th2 infection.
(A-C) Representative flow cytometric plots of influenza-infected Eomesmcherry/+ (reporting for Eomes mRNA transcript) or C57BL/6 mice. Splenic germinal center B cells (A, B), plasma cells (A, C) and CD8 T cells (B-C) were assessed for Eomes expression 8 days post-infection. CD8 T cells from C57Bl/6 mice are represented by the dotted line. (D) Representative flow cytometric plots of mice that were infected with T. muris and assessed for Eomes expression in germinal center B cells and CD3+ T cells 21 days post-infection. Eomesmcherry/+ data are representative of ≥3 mice per experiment.