Klf5 suppresses ERK signaling in mouse pluripotent stem cells
Fig 3
Klf5 regulates Spred1 in mouse ESCs.
(A) RT-qPCR analysis of genes involved in the FGF-FGFR-ERK pathway. (B) Binding peaks of Klf2, Klf4, and Klf5 to Spred1 locus. Numbers below binding peaks indicate regions for ChIP primers. (C) Manual ChIP assay of Klf2, Klf4, and Klf5 binding to Spred1 in mouse ESCs. (D) Spred1 mRNA expression in Klf5-KO ESCs overexpressing Klf2, Klf4, or Klf5. Asterisks indicate statistical significance: *P < 0.01, P < 0.001** (Mann-Whitney U test).