The minor gentamicin complex component, X2, is a potent premature stop codon readthrough molecule with therapeutic potential
Fig 1
Gentamicin complex and individual components cause readthrough of a native p53-NS allele in HDQ-P1 cells.
p53 was detected using a previously described MSD immunoassay (A and B) or by western blot hybridization analysis (C). (A) Readthrough activity of gentamicin complex components is similar to that of the gentamicin complex. (B) Gentamicin X2 and G418 are potent readthrough compounds as compared to the gentamicin complex. Data in Fig 1A and 1B are from a representative experiment. Summarized data from all readthrough experiments can be found in Table 1.