Calorie restriction does not influence oocyte quality in oocytes from POLG mitochondrial mutator mice
Fig 2
CR does not improve chromosome alignment or meiotic spindle assembly in oocytes from POLGD257A/257A mice.
A. Representative laser scanning confocal micrographs of meiotic spindles in MII oocytes from WT, 7-month-old POLGD257A/257A, and POLGD257A/257A-CR mice; α-tubulin labeling of spindle (green) and DNA labeled with DAPI (blue). Scale bars = 5 μm. B, C. Percentage of chromosome misalignment and abnormal spindle assembly in oocytes from WT, POLGD257A/257A, and POLGD257A/257A-CR mice collected at 3–9 months of age (mean shown, error calculated as SD of a binomial distribution; only MII analyzed, oocytes were from 3–6 mice per group; N.S., not significant; P > 0.05; * P < 0.05).