In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter
Fig 7
Visualisation of the binding of chimeric anti-MOMP antibodies in seed extract with Campylobacter isolates.
(A, B, C, J, K, L) C. jejuni strain KC40, (D, E, F, M, N, O) C. jejuni strain Cam12/0156 and (G, H, I, P, Q, R) C. coli strain K43/5. Binding with the different Campylobacter isolates is shown with seed extract containing (A, D, G) Nb5-IgA, (B, E, H) Nb23-IgA, (C, F, I) V1-IgA, (J, M, P) Nb5-IgY, (K, N, Q) Nb23-IgY and (L, O, R) V1-IgY. As a negative control, the nanobody V1 against F4-fimbriated E. coli was used. Bright field microscopy was used for the visualisation of the corresponding bacterial cells.