LDH-A regulates the tumor microenvironment via HIF-signaling and modulates the immune response
Fig 2
Effects of LDH-A depletion on intra-tumoral host cells, CD3+, CD4+ T cells, CD31+ blood vessels, F4/80+ TAMs, necrosis.
H&E staining and immunofluorescence (IF) imaging of CD31+ vessels, CD3+ T cells, CD4+ T cells and TAMs (using F4/80 as a macrophage marker); scale bar, 100 μm (A). Quantitative analysis of blood vessel density (% area covered by CD31 staining), and density of CD3+ and CD4+ T cells and F4/80+ TAMs density using MetaMorph software (thresholding images) and counting the number of T-cells or TAMs (#/mm2). Statistics: *p< 0.05; **p<0.01, ***p< 0.001, (B). H&E staining was performed. The extent of necrosis was assessed using ImageJ. Statistics: **p<0.01 (A).