Decreased nitrite reductase activity of deoxyhemoglobin correlates with platelet activation in hemoglobin E/ß-thalassemia subjects
Fig 1
Decreased HbNO production in HbE/ß-thal subjects.
(A) HbNO production from the reaction between 0.5 μM nitrite and deoxyHb dialysates (50 μM heme) of normal subjects (AA, n = 10) and non-splenectomized HbE/ß-thal subjects (n = 10) as a function of time. HbNO was measured by the chemiluminescence method. *P < 0.001 by unpaired t-test. Data are means ± SD. (B) Rate of HbNO production was measured at 30 seconds from the reactions between 0.5 μM nitrite and deoxyHb (50 μM heme) dialysates from normal subjects (n = 47), non-splenectomized HbE/ß-thal subjects (n = 58), and splenectomized HbE/ß-thal subjects (n = 23). *P < 0.0001, one-way ANOVA with Bonferroni post hoc test. Lines represent means ± SD.