Transvection-like interchromosomal interaction is not observed at the transcriptional level when tested in the Rosa26 locus in mouse
Fig 5
Expression of endogenous genes in knock-in mice.
(A and B) Schematic representation of combinations of enhancer/promoter knock-in alleles to test for intra- and interchromosomal enhancer-promoter interactions in the adult spleen (A) or fetal liver (B) cells. (C-J) The adult spleen was analyzed by qRT-PCR. In addition to the β(γ) promoter alleles, animals used in the panels C-F and G-J carried the LCR or the LCR+β(ε) enhancer alleles (shown in A), respectively. (K, L) The fetal liver of the animals bearing the LCR+β(ε) enhancer allele (shown in B) was analyzed by qRT-PCR. The ratios of Thumpd3 and Setd5 genes expression to that of the GAPDH gene (C, D, G and H) or those of β-major- and βh1-globin genes expression to that of the α-globin gene (E, F, I, J, K and L) were calculated and average value with S.D. was graphically depicted. Although values are arbitrary, they can be quantitatively compared between the panels. Sample numbers analyzed in the panels C-F, G-J and K-L are 28, 13 and 16, respectively, in each group.