Cardiomyocytes cultured on mechanically compliant substrates, but not on conventional culture devices, exhibit prominent mitochondrial dysfunction due to reactive oxygen species and insulin resistance under high glucose
Fig 6
Effect of high-glucose treatment in presence or absence of ROS scavenger on insulin-stimulated glucose uptake and glucose transporter expression.
Cardiomyocytes were seeded either on glass coverslips (Glass) or 15 kPa PAA gels (15 kPa). Cells were exposed to 5 or 15 mM glucose for 24 h in the absence or presence of 1 mM NAC. (A) Insulin-stimulated glucose uptake was measured as described in Materials and methods. Data are expressed as mean ± SD (n = 5). (B) Expression of GLUT1, GLUT4 and β-actin (ACTB) was quantified by real time PCR as described in Materials and methods. The ratio of GLUT1 (Left panel) or GLUT4 (Middle panel) expression to β-actin expression was evaluated, and the ratio of GLUT1/ACBT or GLUT4/ACTB observed in heart tissue isolated from three neonatal rats was set as 100%. (Right panel) The ratio of GLUT4 expression to GLUT1 expression is shown. Data are expressed as mean ± SD (n = 5). *p < 0.05, **p < 0.01.