A set of conformationally well-defined L/D-peptide epitopes provides a serological bar code for autoantibody subtypes
Fig 4
HSQC spectrum excerpt of bi-disulfide peptide 2md (c1/c14,C5-C10).
The 1JC,H correlations between the β-carbons of all cysteines and their corresponding protons are shown. They prove a reduced state for c1 and c14 (~25 ppm, low 1H signal dispersion) and a disulfide bridge between C5 and C10 (over 40 ppm, high 1H signal dispersion; 600 MHz, 280 K, 50 mm potassium phosphate buffer (pH = 3.0)/D2O 9:1).