Altered hepatic glucose homeostasis in AnxA6-KO mice fed a high-fat diet
Fig 1
Liver function, lipid and insulin-regulated glucose metabolism are normal in chow-fed AnxA6-KO mice.
(A) Spider diagram presentation of total protein, albumin, aspartate aminotransferase (AST), alanine aminotransferase (ALAT), lactate dehydrogenase (LDH), as well as total cholesterol, triglycerides (TAG), low density lipoprotein (LDL), high density lipoprotein (HDL), and free fatty acids (free FA) in the plasma of 8–12 weeks old wildtype (WT) and AnxA6-KO animals (AnxA6-/-) on a chow diet (n = 4). The data are presented relative to WT (100%). For absolute values, see text and [28] for further details. (B) H&E staining of liver sections from WT and AnxA6-KO mice on a chow diet. (C) Liver homogenates from 8–12 weeks old WT and AnxA6-KO mice were analyzed for triglyceride, cholesterol and phospholipid content as described in Material and Methods (mean ± SEM, n = 8). SM, sphingomyelin; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PS, phosphatidylserine. (D) WT and AnxA6-KO mice on a chow diet were starved for 4 h prior to receive glucose via oral gavage (1 g/kg body weight). Blood glucose levels were determined 0–120 min after glucose administration (mean ± SEM, n = 6). (E) WT and AnxA6-KO mice received insulin (1.5 U/kg bodyweight) or saline via intraperitoneal injection. Blood glucose was measured at t = 0–120 min post-injection (mean ± SEM, n = 6). (D-E) Data points for t = 0 and 30 min have been described previously [28].