Inhibition of endocytosis suppresses the nitric oxide-dependent release of Cl- in retinal amacrine cells
Fig 3
Dynamin inhibitors are effective in limiting activity-dependent FM1-43 labeling.
A-D, Mean percent fluorescence change for cell bodies and processes for each of the dynamin inhibitors tested. Dynasore (but not the other inhibitors) increased baseline fluorescence such that after the voltage steps, the FM1-43 fluorescence was lower than control values giving rise to negative percent change in fluorescence. (Cell bodies: control n = 6; dynasore n = 6, p < 0.0001; Dyngo 4a n = 10, p < 0.0001; Dynole 34–2 n = 10, p < 0.0001; MiTMAB n = 6, p < 0.0001. Processes: control n = 6; dynasore n = 12, p < 0.0001, Dyngo 4a (30 μM), n = 12, p < 0.0001; Dynole 34–2 (10 μM) n = 10, p < 0.0001; MiTMAB (30 μM) n = 12, p < 0.0001, unpaired t-tests).