Inhibition of endocytosis suppresses the nitric oxide-dependent release of Cl- in retinal amacrine cells
Fig 2
Voltage steps elicit endocytosis.
A, Perforated-patch recording from a representative AC. The voltage was stepped from -70 mV to +10 mV for 100 msec and delivered 15 times with a 3 second delay between the steps. Currents resulting from all 15 voltage steps are shown. (Bottom) Scale bars are 100 pA and 50 ms. B, Another AC being recorded in the perforated patch configuration. C, Cells were exposed to the lipophillic dye FM1-43 (4 μM) for two minutes then imaged to establish a baseline control. D, An image after the series of voltage steps. Labelling can be seen in the cell body and in processes. Scale bars are 15 μm. E, Mean % fluorescence change ± SD. (paired t-test: cell bodies n = 6, p < .05; processes n = 8, p < .05).