Enteral administration of bacteria fermented formula in newborn piglets: A high fidelity model for necrotizing enterocolitis (NEC)
Fig 5
Fig 5A and 5B: Apoptotic index: Apoptotic cells were determined by TUNEL. Representative TUNEL-positive apoptotic cell is exhibited by dark brown color (Fig 5A). Three images represent different treatment in newborn piglets. The percentages of apoptotic cells in each group are presented in bar graph from quantification of images (Fig 5B). Fig 5C and 5D: Pro-apoptotic proteins: Intestinal cleaved caspase-3 (Fig 5C) and BAX (Fig 5D) protein expressions in each group were determined by Western blot. Cleaved caspase-3 levels in FF and BO groups were significantly increased compared to FO group. Higher levels of cleaved caspase-3 in FF group were noticed compared to BO group. BAX protein in FF group significantly increased compared to BO and FO groups. The data are represented as mean ± SE (* P<0.05, **P<0.01, N = 5–8 / group).