Enteral administration of bacteria fermented formula in newborn piglets: A high fidelity model for necrotizing enterocolitis (NEC)
Fig 3
p65 NF-κB and TLR4 activation and expression.
Intestinal p65 NF-κB activation in each group was assayed by EMSA. NF-κB mRNA was determined by qRT-PCR. P65 NF-κB and TLR4 proteins were measured by Western blot. Fig 3A: Image and bar graphs showed TLR4 protein expressions. Significant increase in TLR4 protein were seen in FF and BO groups. Fig 3B: Intestinal NF-κB mRNA expression levels. NF-κB mRNA levels in FF group were higher than BO and FO groups. Fig 3C: Intestinal p65 NF-κB protein levels. The levels of p65 NF-κB protein from FF group were higher than BO and FO groups. Fig 3D: Bar graphs, obtained by densitometric analysis of EMSA (the images not shown). The DNA binding activities in FF and BO groups were significantly increased compared to FO group. The data are represented as mean ± SE (* P<0.05, **P<0.01, N = 5–8 / group).