Expansion of the TLO gene family enhances the virulence of Candida species
Fig 8
Growth of C. dubliniensis ΔΔtlo and derivatives harboring C. albicans TLO genes.
A and B show doubling times of WT Wü284, the ΔΔtlo double mutant and derivatives expressing indicated C. albicans TLO genes in YEP-Glucose (A) and -Galactose (B). Stars indicate strains exhibiting doubling times significantly different from ΔΔtlo (p ≤ 0.05). Panel C shows biofilm formation on plastic surfaces. Each ΔΔtlo::TLO strain was grown in the presence of YEPD in a 96-well plate for 48 h. Biomass was measured using a crystal violet assay in three replicate experiments. Asterisks indicate significant differences from ΔΔtlo at 24 h (*) and 48 h (red *), respectively. Data are the result of three independent experiments.