Effects of lobeglitazone on insulin resistance and hepatic steatosis in high-fat diet-fed mice
Fig 6
Effect of lobeglitazone (Lobe) on serum leptin and hypothalamic signaling transducer and activator of transcription 3 (STAT3) and proopiomelanocortin (POMC) 2 expression in HFD-fed mice.
(A) Serum leptin concentrations and (B) western blot and quantification of p-STAT3 and total STAT3 in the hypothalamuses from each group. Densitometry values for each protein are normalized to total STAT3. (C) Representative microphotographs of immunostained POMC-positive cells in the arcuate nucleus of hypothalamuses from each group. Asterisks indicate POMC-positive neurons. Scale bar = 50 μm. (D) The number of POMC-positive cells. Data are presented as mean ± SEM. *P <0.05 vs. ND-fed mice; †P <0.05 vs. HFD-fed mice. HFD, high-fat diet; Lobe, lobeglitazone; ND, normal diet.