Dopamine and its receptors play a role in the modulation of CCR5 expression in innate immune cells following exposure to Methamphetamine: Implications to HIV infection
Fig 9
Surface expression of CCR5 in the presence of selective DRD isoform agonists.
Control (A, B, C, D, I, J, K, L) and Meth-treated (E, F, G, H, M, N, O, P) THP1 cells were exposed for 1 hour to DRD1–5 agonist SKF 38393 (A, B, E, F), DRD2 agonist (C, D, G, H), DRD3 agonist Pramipezole (I,J, M, N) and to DRD4 agonist PD 168077 (K, L, O, P). The cells were stained with PE- labeled CD11b and APC-labeled CCR5. Scatter plots show the superposition of CCR5 and CD11b subsets in non-treated cells (NT), and cells treated with antagonists in each panel’s legend, with or without simultaneous Meth (60uM). (Q) CCR5 levels measured by the geometric mean fluorescence of cells that received the different DRD agonists, in the presence or absence of Meth. The results represent the average ± SD of 3 independent experiments performed in duplicate. *p<0.05 in Bonferroni’s test, and in comparison to respective controls, or white bars are compared to not treated (NT) without Meth, and black bars are compared to Meth alone NT controls.