CRISPR/Cas-based customization of pooled CRISPR libraries
Fig 3
High-throughput screening with the HPRT1-depleted libraries.
(A) Relative HPRT1 gRNA quantities were calculated from the targeted deep sequencing data on the cells infected with each library. Error bars indicate SEM. **P < 0.01, ***P < 0.001. Student’s t-test. (B) Crystal violet staining images after 6-TG selection of cells infected with designated libraries. 6-TG-resistant clones were observed only in the cells infected with the wild-type libraries. (C) Log2-fold change in gRNAs in cells infected with the wild-type or the HPRT1-depleted GeCKOv2 A library after 6-TG selection. All three HPRT1 gRNAs were highly enriched in the cells infected with the wild-type library. Numerical data are presented in S1 Table.