Discovery of microRNA-like RNAs during early fruiting body development in the model mushroom Coprinopsis cinerea
Fig 3
Validation of two milRNA candidates by northern blot and RT-qPCR.
MYC: mycelium, PRI: primordium. Northern blot of sRNA samples showed the presence of (a) cci-milR-12c and (b) cci-milR-13e-5p in both developmental stages of C. cinerea. The top panel shows northern blots probed with the milRNA-specific DIG probes. The 15% denaturing gel stained with ethidium bromide (EtBr) in the bottom panel indicates equal loading of RNA samples. RT-qPCR results show the expression levels of (c) cci-milR-12c and (d) cci-milR-13e-5p in both developmental stages. Results were obtained from three independent experimental replicates and were significantly different between stages. ** p < 0.01, *** p< 0.001.