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First record of in vitro formation of ectomycorrhizae in Psidium cattleianum Sabine, a native Myrtaceae of the Brazilian Atlantic Forest

Fig 1

Schema representing the bioassay of the in vitro association between Psidium cattleianum (Red Araza) and ectomycorrhizal fungi D17 and UFSC-Sc133.

(A) Methodology for the symbiosis bioassay. (B) Test tubes containing vermiculite and Red Araza seedlings, where: Ct, control (no fungi added); Sc, treated with mycelium from the UFSC-Sc133 (Scleroderma citrinum) isolate; and Pi, treated with mycelium from the D17 (Pisolithus microcarpus) isolate. *Modified Melin-Norkrans Medium [27] containing 10 g of glucose, 5 g of sucrose, and 200% of the micronutrients from an MS0 medium [28]. Squares were dimensioned at 1 cm by 1 cm.

Fig 1

doi: https://doi.org/10.1371/journal.pone.0196984.g001