β-Arrestin1 and 2 differentially regulate PACAP-induced PAC1 receptor signaling and trafficking
Fig 5
Differential effects of silencing of β-arrestin1 and β-arrestin2 on PACAP-induced ERK1/2 activation.
(A) Representative images of western blots for total and phosphorylated ERK1/2 in HEK293T cells transfected with the indicated siRNA and treated with 1 μM PACAP or saline for 3 or 25 min. (B) Quantification of ERK1/2 activation by normalizing phosphorylated ERK1/2 to total ERK1/2 levels analyzed by western blotting. β-arr1, β-arrestin1; β-arr2, β-arrestin2. Values are mean ± SEM of three independent experiments. *p < 0.05 and **p < 0.01, two-way ANOVA followed by Fisher-PLSD test.