High throughput generation and characterization of replication-competent clade C transmitter-founder simian human immunodeficiency viruses
Fig 3
Infectivity of various SHIVs were measured using β-gal assay.
TZMbl cells were infected with 200TCID50 of 293T cell generated viral supernatant using standard TZMbl assays as described previously. After 72-hrs post infection β-gal assay was measured and Infectious units are calculated. Full-length SHIVs env 375S (panel A) with env 375H (panel C) and env 375Y (panel E). Similarly, truncated SHIVs env 375S (panel B) with env 375H (panel D) and env 375Y (panel F).