Cell iron status influences macrophage polarization
Fig 4
Iron deficiency increases LPS-induced IL-12/IL-10 balance in vivo.
C57BL/6 wild type mice were either fed with iron replete diet (IR) or iron-deficient diet for 14 days (Low) or iron-rich diet for 3 days (High), or treated with iron-dextran (Fe-dextran, 0.2 g/kg ip) for 48h. Mice were then treated with LPS (i.p 50 μg/kg) or NaCl 0.9% as vehicle for 4 h. Peritoneal cells were collected to assess the Il-12, Il-10, and Il-1β gene expression using quantitative PCR. (A) data from mice fed with various diets and (B) comparison of mice fed with high iron diet or treated with iron-dextran. Data are expressed as mRNA fold change relative to vehicle control mice under IR diet. Gene expression was then analyzed using quantitative PCR analysis. Data are expressed as mRNA fold change relative to control mice fed with IR diet. Data are from two independent experiments and presented as mean ± SD (n = 6 mice per group). ns, non significant; ** p≤ 0.01; *** p≤ 0.001.