Multiplexed bovine milk oligosaccharide analysis with aminoxy tandem mass tags
Fig 3
MS/MS spectrum of lacto-N-tetraose in profile (upper) and centroid (lower) formats.
Resolution, defined as the quotient of m/z and peak width, or m/Δm, of the TMT6-126 reporter ion and the HexNAc oxonium ion at m/z 126.055 are sufficient for successful differentiation of the two peaks. The instrument provides near baseline separation of the ions in the profile data and allows successful conversion to centroid format.