UXT is required for spermatogenesis in mice
Fig 2
Uxt knockout suppresses germ cell expansion between 6 and 7 days post-partum (dpp).
(A) IHC for TRA98 in representative tissue sections at 6dpp (left column) and 7dpp (right column) in Uxt F/Y and Uxt F/Y; Vasa-Cre testes. (B) Mean number of TRA98-positive cells per tubule cross-section at 6dpp (n = 5; p = 0.64) and 7dpp (n = 5; *p = 0.024) in Uxt F/Y (WT) compared to Uxt F/Y; Vasa-Cre (KO) testes. (C) IHC for PLZF in representative tissue sections at 6dpp (left column) and 7dpp (right column) in Uxt F/Y and Uxt F/Y; Vasa-Cre testes. (D) Mean number of PLZF-positive cells per tubule cross-section at 6dpp (n = 7; p = 0.65) and 7dpp (n = 5;* p = 0.036) in Uxt F/Y compared to Uxt F/Y; Vasa-Cre testes. The combined average number of positive cells per tubule from n mice is plotted. Error bars represent the standard error of the mean (S.E.M.). Unpaired Student’s t-tests were performed for all samples. Scale bars on the upper left micrographs are 50 microns.