Proteomic analysis of human epileptic neocortex predicts vascular and glial changes in epileptic regions
Fig 1
Pairs of tissues from low and high spiking neocortical brain regions are removed as part of the patient’s clinically-determined surgical procedure to treat refractory epilepsy. Each tissue sample is homogenized and separated by centrifugation into three subcellular fractions (Cytosolic, Nucleic and Membrane) and analyzed by 2D-DIGE. Spots found to vary were identified by LC-MS/MS. The proteomic information was used in pathway analyses.