Bacterial ghost of avian pathogenic E. coli (APEC) serotype O78:K80 as a homologous vaccine against avian colibacillosis
Fig 2
(A) The sensitivities of the wild type E. coli O78:K80 to ampicillin 10, Chloramphenicol 30, kanamycin 30, sulfametoxazol 100, gentamicin 10 and Neomycin 30 discs are shown; (B) amplification of gene aacC1 from plasmid pCOM8. The two indicated bands are representative of aacC1 gene amplified by PCR reaction. The ladder size is 100bp; (C) Gel electrophoresis of restriction enzyme digestion products for plasmid pmET32b: pmET32b was digested with two different sets of restriction enzymes “Xhol, BspHI” and “HindIII”. Products related to each set of digestion are shown. 1Kbp ladder was used.