The Arabidopsis thaliana Mediator subunit MED8 regulates plant immunity to Botrytis Cinerea through interacting with the basic helix-loop-helix (bHLH) transcription factor FAMA
Fig 4
FAMA overexpression plants enhance B. cinerea resistance.
(A) RT-qPCR of FAMA at 6 d after germination of FAMA overexpression lines (OE-3 and OE-7) and WT using Actin7 as a control. Error bars indicate 95% confidence intervals (n = 3). (B) Localization of FAMA in OE-3 and OE-7 leaves. Confocal imaging of transgenic A. thaliana plants expressing FAMA. Bars = 20 μm. (C) (D) Disease symptoms and lesion sizes on the B. cinerea-infected WT, OE-3, and OE-7 leaves. (E) Trypan blue staining of B. cinerea fungal hyphae growing on leaves at 3 d. Bars = 100 μm. (F) Fungal growth on the B. cinerea-infected WT, OE-3, and OE-7 leaves. The disease assay was performed as indicated (see Results and Methods) on the leaves of soil-grown plants. Photos (C) were taken at 3 d. Bars = 3 mm. Fungal growth in planta was assumed by analyzing the transcript levels of the BcActinA gene by RT-qPCR using Actin7 as an internal control 3 d after inoculation (F). Average values and SEM from relative values obtained in three biological replicates were plotted on the graph (D) (F). A minimum of 10 leaves for each genotype was used for each biological replicate, and the disease assay was repeated three times, with similar results. The mean values followed by different letters represent significant differences (P< 0.01, Student’s t-test).