Purification and characterization of the first γ-phospholipase inhibitor (γPLI) from Bothrops jararaca snake serum
Fig 1
A) Fractionation of B. jararaca serum on ion exchange column (DEAE). Elution was initially done keeping buffer A (25 mM Tris, pH 7.5), 10% of buffer B (25 mM Tris, 1M NaCl pH 7.5) and then making a gradient of B up to 50% (500 mM NaCl), with a flow rate of 1 mL/min. B) Elution of the fractions of pool D2 applied to an affinity column (CNBr-activated Sepharose + crotoxin), done with 1 M glycine pH 2 (indicated by an arrow). SDS-PAGE: 1. Molecular mass marker (Dual Color Precision Plus, BioRad) 2. γBjPLI with β-mercaptoethanol; 3. γBjPLI without β-mercaptoethanol.