Pre-exposure to 50 Hz-electromagnetic fields enhanced the antiproliferative efficacy of 5-fluorouracil in breast cancer MCF-7 cells
Fig 3
Pre-exposure to 50 Hz-EMFs enhanced the antiproliferative efficacy of 5-FU in breast cancer cell line MCF-7.
(A) For EMF exposure time assay, MCF7 cells were exposed to 50 Hz-EMF (1 mT) for 0, 2, 4, 8 and 12 h; then, the cells were treated with 5 μM 5-FU for 24 h, and cell viability was analyzed by the MTT assay. (B) For 5-FU concentration assay, MCF7 cells were exposed to 50 Hz-EMF (1 mT) for 12 h; then, the cells were treated with 1, 2.5, 5 or 10 μM 5-FU for 24 h, and cell viability was analyzed by the MTT assay. (C) MCF7 cells were exposed to 50 Hz-EMF (1 mT) for 12 h; then, the cells were treated with 5 μM 5-FU for 24 h, and cell apoptosis was measured by flow cytometry. (D) and (E) MCF10A cells were subjected to the same treatment as in (A) and (B), respectively. n = 3, *p < 0.05, **p < 0.01.