Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with the ability to include or exclude detection of Staphylococcus argenteus
Fig 3
Nuc amplification of CCUG31966 and CC75-08 achieved in hydrolysis probe PCR.
(a) S. aureus (CCUG31966) amplified with nuc-F+nuc-R1+nuc-R2 and nuc-F+nuc-R2. (b) CC75 lineage strain/S. argenteus (CC75-08) amplified with nuc-F+nuc-R1+nuc-R2 and nuc-F+nuc-R1.