Is oxygen availability a limiting factor for in vitro folliculogenesis?
Fig 3
Viability of bovine follicles in strips cultured in air in PDHV vs CDHV.
Representative confocal micrographs of a live (A,B,C) and a dead (D,E,F) follicle. (A,D) Hoechst 33342–stained nuclei; (B,E) live–dead far-red probe; (C,F) merge. (G) Follicle viability in fresh and cultured strips. Arrowheads indicate a dead oocyte. Bar = 20μm. *P<0.01 vs d0; #P<0.01 vs corresponding treatments.