Semaphorin3A induces nerve regeneration in the adult cornea-a switch from its repulsive role in development
Fig 3
Sema3A does not inhibit the NGF induced neuronal growth of adult DRG and TG neurons.
Isolated DRG and TG neurons were incubated with NGF for 3 and 2 days respectively to induce neuronal growth and then treated with different concentrations of Sema3A to determine any significant growth inhibitory effect on these PNS neurons. Neurite growth was evaluated 24 h after addition of Sema3A. (A) We found that about one third of the isolated neurons responded to the NGF treatment and addition of Sema3A produced no inhibitory effects such as the neurons kept growing similarly as compared to control cells treated with NGF alone. (B) TG neurons responded to NGF similarly to DRG neurons and addition of Sema3A did not alter the growth of neurites and no axonal retraction was observed. Neuronal growth was similar to controls that were resupplied with NGF over the course of the experiment. Values represent mean ± SEM, experiments were performed in triplicate, each dish had an average of 200 neurons for DRG and 60 neurons for TG; all neurons in every dish were evaluated.