A trivalent Apx-fusion protein delivered by E. coli outer membrane vesicles induce protection against Actinobacillus pleuropneumoniae of serotype 1 and 7 challenge in a murine model
Fig 13
Lymphocyte proliferation assay.
Levels of lymphocyte proliferation were detected using an MTS method and the results shown as absorbance at 490 nm. Splenocytes of mice two weeks post boost immunization were isolated and stimulated with recombinant Apxr protein and con A.