Direct exposure to mild heat promotes proliferation and neuronal differentiation of neural stem/progenitor cells in vitro
Fig 6
Phospho-Akt (p-Akt) upregulation in neural stem/progenitor cells (NSCs/NPCs) by mild heat exposure.
Representative blots (A) and mean relative blot density (B) showing expression of phospho-Akt in NSCs/NPCs on day 1 and day 4. The blot intensity of phospho-Akt in each group was normalized to that of β-actin and the values at 37.0°C were set to 1.0. Values are mean ± SEM of three independent experiments. *P < 0.05 vs. control temperature. 6 (C) Viability of NSCs/NPCs at 37.0°C and 38.5°C in the presence of the PI3K inhibitor LY294002 (0−50 μM) or vehicle (DMSO). Viability of cells in DMSO at 37.0°C was taken as control, and data are expressed as percentages of the control condition. Results are mean ± SEM of three independent experiments. *P < 0.05 vs. control condition.