Vibrio cholerae O1 secretes an extracellular matrix in response to antibody-mediated agglutination
Fig 7
ECM production by V. cholerae C6706 in response to ZAC-3 IgG is VPS independent.
Mid-log phase cultures of either V. cholerae C6706 El Tor or an isogenic vpsL mutant were treated for 1 h at 37°C in static conditions with ZAC-3 (9μg/mL) or an isotype control MAb. Both strains displayed, as compared to isotype controls, increased levels of CV straining in response to ZAC-3 IgG, as determined by a two-way ANOVA followed by a Tukey multiple comparison test. *; P< 0.05. The results presented are the average of at least three biological replicates with three technical replicates each. (B) ECM-ELISA of the same treatment groups described above, with no significant difference in VPS signal seen between the control and ZAC-3 treated groups in either strain, as determined by two-way ANOVA followed by a Tukey multiple comparison test. Graph is composed of data from two biological replicates with 2 technical replicates each.