Vibrio cholerae O1 secretes an extracellular matrix in response to antibody-mediated agglutination
Fig 4
Relationship between agglutination and ECM production by V. cholerae O395.
CV staining induced following treatment (2.5 h) with (A) ZAC-3 Fab fragments, (B) 2D6 IgA, (C) 2D6 IgG and (D) ZAC-3 IgG at the indicated concentrations (y-axis). Note that panel (D) is reproduced from Fig 2A for the sake of comparison. Appropriate isotype and Fab controls (50 μg/mL) were included in each experiment and are shown by solid black bars. Vertical symbols indicate macroagglutination (Agglut.);—no agglutination; +++ agglutination within 1 h; ++ agglutination within 2 h, and + agglutination within 3 h. Each graph constitutes at least three biological replicates with three technical replicates each. Statistical significance across treatments was determined by one way ANOVA, followed by a Tukey multiple comparison test, *; P< 0.05. ns; not significant.