Effects of mechanical repetitive load on bone quality around implants in rat maxillae
Fig 5
Effects of mechanical repetitive load on collagen fibers inside and outside area of threads A and B (A) Representative images of picrosirius red-stained sections with polarized microscopy and emphasized images in thread A (Bar: 100 μm; green and yellow-orange indicate type III and type I collagen, respectively). (B) Type I collagen area fraction was significantly smaller inside thread A vs. outside thread A at 200–400 μm away from implants under non-loaded conditions. (C) Type III collagen area fraction was similar, irrespective of analyzed areas under non-loaded conditions. (D) Type I collagen area fraction was higher in the loading vs. control group, but the difference was not significant. (E) Type III collagen area fraction was significantly higher in the loading vs. control groups. (F) Representative images of picrosirius red-stained sections with polarized microscopy and emphasized image in thread B. (Bar: 100 μm; green and yellow-orange represent type III and type I collagen fibers, respectively) (G) Type I collagen area fraction was smaller inside thread B vs. outside thread B at 200–400 μm away from implants under non-loaded conditions. (H) Type III collagen area fraction was smaller inside thread B vs. outside thread B at 0–200 μm and 200–400 μm away from implants under non-loaded conditions. (I) Type I collagen area fraction was significantly higher in the loading vs. control group. (J) Type III collagen area fraction was significantly higher in the loading vs. control groups. (K) Angle differences of collagen alignment to the long axis of implants were similar among groups under non-loaded conditions. (L) Representative images of the preferential alignment of collagen fibers in thread A (yellow represents collagen fibers and double arrowheads indicate the preferential alignment of collagen fibers). (M) Angle difference of collagen alignment in the upper flank of thread A was similar between groups. (N) Angle difference of collagen alignment in the lower flank of thread A was similar between groups. (O) The angle difference of collagen alignment to the long axis of implants inside thread B was bigger than that outside thread B, but the difference was not statistically significant. (P) Representative images of the preferential alignment of collagen fibers in thread B. (Q) Angle difference of collagen alignment in the upper flank angle of thread B was the same between groups. (R) Angle difference of collagen alignment in the upper flank angle of thread B was significantly smaller in the loading vs. control groups. (Bar: 100μm) (n = 7 each, *p<0.05, **p<0.01).