Impact of diabetes on gingival wound healing via oxidative stress
Fig 2
Histological and histomorphometric analysis.
(A) Representative histological photographs. Note that on day 21 the wounds were completely closed and inflammatory cells were lost in the control group. While, the wounds were still not closed (arrow head) and infiltrating of inflammatory cells were not remained (asterisk) in the diabetes mellitus (DM) group. Hematoxylin–eosin staining, magnification ×40. Scale bar: 50 μm. (B) Histomorphometric analysis of wound site. The areas of epithelium (a) and connective tissue (b) were measured in the enclosed area with horizontal lines and vertical lines from the arrowhead (Δ). The re-epithelialization rate was calculated as (a / a + b). (C) Re-epithelialization rates in the control and DM groups. On days 3 and 7, delayed re-epithelialization was observed in the DM group. Data are presented as means ± SD (n = 6). *p < 0.05 (t-test).