Whole genome resequencing identifies the CPQ gene as a determinant of ascites syndrome in broilers
Table 1
Primers, probes, and conditions for RT-qPCR.
For each CPQ locus: position is the [chromosome]:[chromosomal position] of the 5’ base according to the Gallus_gallus-5 assembly; Primers are 5’-3’ for forward (F) and reverse (R); Probes are 5’-3’ with allele 1 (P1) labeled with FAM and allele 2 (P2) labeled with HEX. Annealing temperatures for qPCR are indicated in °C.