Effects of genetic polymorphisms on the OCT1 and OCT2-mediated uptake of ranitidine
Fig 2
Effects of OCT1 polymorphisms on ranitidine uptake.
A) OCT1 alleles analyzed in this study. Amino acid substitutions known to affect OCT1 function are highlighted in yellow. Amino acid substitutions known to not significantly affect OCT1 function are highlighted in white. B) to E) Effects of OCT1 allelic variants on ranitidine uptake. HEK293 T-REx™ (B, C, E) and CHO (D) cells overexpressing the reference allele (OCT1*1B) and common OCT1 allelic variants (the allelic variants are listed in panel A) were incubated with 1 μM (B to D) or with increasing concentrations (E) of ranitidine for 2 min. The OCT1-mediated uptake was calculated by subtracting the uptake of control cells from the uptake of OCT1-overexpressing cells. The data is shown as means and standard error of the means of at least three independent experiments.