Characterization of a candidate tetravalent vaccine based on 2'-O-methyltransferase mutants
Fig 4
Dengue MTase mutants are immunogenic in monkeys and induce a protective immune response.
A) Kinetics of DENV E protein-specific IgG titer of monkeys immunized with tetravalent MTase mutants formulation or RPMI placebo, respectively. Monkeys were immunized i.d. with a mix of 1×104 PFU of each MTase mutant (total of 4×104 PFU). B) Neutralizing antibody (nAB) titers of monkeys immunized as described above. nAB against all four DENV serotypes were measured by flow cytometry-based neutralization assay. C) 97 days post immunization monkeys were challenged with DENV-2 (1x105 PFU of strain D2Y98P) i.d.. Viremia after challenge was measured by real-time PCR as indicated. Data are representative of one experiment with a total of 3 monkeys per group. Shown are means with SD for all panels. Statistical analysis was performed using Student's t-test (B). *, p < 0.05).