Characterization of a candidate tetravalent vaccine based on 2'-O-methyltransferase mutants
Fig 2
2’-O-MTase mutants are more sensitive to IFN.
A) DENV WT or MTase mutant infection of HEK-DC-SIGN cells pretreated with increasing amount of IFN-β. HEK-DC-SIGN cells were seeded in a 24-well plate and after incubation overnight, pre-treated for 24 hours with the indicated amount of IFN- β. 24 hours after addition of IFN- β, cells were infected at an MOI of 1. B) DENV WT or MTase mutant infection of monocyte-derived dendritic cells (moDCs) pretreated with increasing amount of IFN-β. Means and SD are shown. Statistical analysis was performed using Student's t-test (****, p < 0.0001; ***, p < 0.001; **, p < 0.01; *, p < 0.05). Results shown are from three experiments with n = 4–11 measurements per condition (A) or from two experiments (from two different donors) with n = 4, except for DENV-3 where one experiment is shown from one donor with n = 2 (B).